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AIM:γ-glutamyl transpeptidase(GGT)has been reportedas a virulence and colonizing factor of Helicobacter pylori(H pylori).This study examined the effect of GGT on thegrowth of H pylori.METHODS:Standard H pylori strain NCTC 11637 and 4dinical isolates with different levels of GGT activity as measuredby an enzymatic assay were used in this study.Growthinhibition and stimulation studies were carried out by culturingH pylori in brain heart infusion broth supplemented withspecific GGT inhibitor(L-serine sodium borate complex,SBC)or enhancer(glutathione together with glycyl-glycine),respectively.The growth profiles of H pylori were determinedbased on viable bacterial count at time interval.RESULTS:Growth was more profuse for H pylori isolateswith higher GGT activity than those present with lower GGTactivity.However,in the presence of SBC,growth of H pyloriwas retarded in a dose dependent manner(P=0.034).Incontrast,higher growth rate was observed when GGTactivity was enhanced in the presence of glutathione andglycyl-glycine.CONCLUSION:Higher GGT activity provides an advantageto the growth of H pylori in vitro.Inhibition of GGT activityby SBC resulted in growth retardation.The study showsthat GGT plays an important role on the growth of H pylori.
AIM: γ-glutamyl transpeptidase (GGT) has been reported as a virulence and colonizing factor of Helicobacter pylori (H pylori). This study examined the effect of GGT on the growth of H pylori. METHODS: Standard H pylori strain NCTC 11637 and 4 medical isolates with different levels of GGT activity as measured by an enzymatic assay were used in this study. Growth inhibition and stimulation studies were carried out by culturing H pylori in brain heart infusion broth supplemented with specific GGT inhibitor (L-serine sodium borate complex, SBC) or enhancer (glutathione together with glycyl-glycine), respectively. The growth profiles of H pylori were determined on either viable bacterial count at time interval .RESULTS: Growth was more profuse for H pylori isolates with higher GGT activity than those present with lower GGT activity. However, in the presence of SBC, growth of H pylori was retarded in a dose dependent manner (P = 0.034) .Incontrast, higher growth rate was observed when GGT activity was enhanced in the presence of glutathione andglycyl-glycine.CONCLUSION: Higher GGT activity provides an advantage to the growth of H pylori in vitro. Inhibition of GGT activity by SBC resulted in growth retardation. The study show that GGT plays an important role on the growth of H pylori.