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作者在该研究中从感染猴免疫缺陷病毒( SIV) Gag Vab T2 52 - 51 重组痘苗病毒( r Vac/SIV Gag)的Hela细胞中经DNA体外扩增得到SIVmac2 39Gag序列,并将此序列设计为5个不同的Gag基因片段( SIV Gag no.l～5) ,通过Nhe 和Hpa 限制性酶切位点插入到嵌合流感( WSN)病毒NA基因的非编
In this study, the authors amplified the SIVmac239Gag sequence from Hela cells of Simian Immunodeficiency Virus (SIV) Gag Vab T2 52-51 recombinant vaccinia virus (SIV) vaccinia virus SIV Gag and designed this sequence as Five different Gag gene segments (SIV Gag no. 1-5) were inserted into the non-coding NA gene of the chimeric influenza (WSN) virus by Nhe and Hpa restriction enzyme cleavage sites