论文部分内容阅读
目的 探讨骨形态发生蛋白 (Bonemorphogeneticprotein ,BMP)诱导成骨过程中骨胶原氨基酸的变化规律及转化生长因子 β1(Transforminggrowthfactorβ1,TGF β1)对骨胶原生成的影响 ,进一步阐明BMP的诱导成骨机制和TGF β1对骨损伤修复的调节机制。方法 实验采用日本大耳白兔左尺骨中上段 12mm骨缺损实验模型 ,随机分为实验组、对照组及空白组 ,缺损内分别植入BMP/TGF β1/载体、BMP/载体及单纯载体。分别于术后第 1、2、3、4、5周检测各组缺损区胶原羟脯氨酸 (Hydroxyproline ,HP)及羟赖氨酸(Hydroxylysine ,HL)量的变化 ,并作胶原VG染色图像分析、组织学及电镜观察。结果 实验组较同期对照组及空白组胶原HP生成量增加 ,成骨细胞数量增多 ,Ⅰ型胶原生成时间提前、生成量增加 ,胶原总量也明显增加 ,骨损伤修复时间缩短。结论 TGF β1可使骨胶原在BMP诱导成骨过程中生成增加。TGF β1通过促进成骨细胞生成使Ⅰ型胶原分泌能力增强 ,并抑制Ⅱ型胶原产生使软骨期缩短 ,骨损伤修复提前。BMP、TGF β1两者在骨损伤修复中相互加强 ,具有协同作用。
OBJECTIVE: To investigate the effect of bone morphogenetic protein (BMP) on bone morphogenetic protein (BMP) induced osteogenesis and the effect of transforming growth factor β1 (TGFβ1) on the formation of collagen in bone, further to elucidate the mechanism of BMP induced osteogenesis and TGF β1 on the repair mechanism of bone injury. Methods The experimental model of 12mm bone defect in the middle and upper left ulna of Japanese white rabbits was randomly divided into experimental group, control group and blank group. BMP / TGFβ1 / BMP, BMP / vector and simple vector were implanted into the defect respectively. The changes of hydroxyproline (HP) and hydroxylysine (HL) in the defect area were detected at 1, 2, 3, 4, and 5 weeks after operation respectively and the collagen VG staining images Analysis, histology and electron microscopy. Results Compared with the control group and the blank group, the amount of collagen HP increased in the experimental group and the number of osteoblasts increased in the experimental group. The generation of type Ⅰ collagen was earlier, the production increased, the total amount of collagen was also increased, and the time of bone injury repair was shortened. Conclusion TGF β1 can increase the production of collagen during BMP-induced osteogenesis. TGFβ1 can enhance type Ⅰ collagen secretion by promoting osteoblastogenesis and inhibit the production of type Ⅱ collagen to shorten the cartilage and repair bone damage in advance. Both BMP and TGFβ1 reinforce each other in the repair of bone injury and have a synergistic effect.