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探索有机磷农药急性中毒的γ -氨基丁酸系统机理 ,为有机磷农药急性中毒的治疗提供理论依据。实验 :用大鼠脑神经末梢微囊GABA受体做模型 ,从大鼠体外实验、体内染毒实验 (动物抽搐发作和不发作 ) ,观察GABA受体的变化。结果 :在体外实验中 ,1× 10 -2 mol/L的二嗪农或丙氟磷能显著降低大鼠GABA受体的结合 (P <0 .0 1) ,1× 10 -3 mol/L的二嗪农仍能显著降低GABA受体的结合 (P <0 .0 5 )。二嗪农 1.0 95 g/kg灌胃大鼠 ,结果发现3 H -GABA的结合值在抽搐发作时显著降低 ,大鼠腹腔注射丙氟磷 6mg/kg ,观察大鼠抽搐发作与不发作 ,3 H -GABA的结合值均显著降低。结论 :有机磷农药在一定浓度下能显著降低大鼠脑GABA受体的结合 ,GABA受体参与了有机磷农药的急性中毒过程
To explore the mechanism of γ - aminobutyric acid system for acute poisoning of organophosphorus pesticides and provide theoretical basis for the treatment of acute poisoning of organophosphorus pesticides. Experiment: Using GABA receptor of rat brain nerve endings microcapsules as a model, we observed the changes of GABA receptor from rat in vitro experiments in vivo (animal convulsions episodes and no seizures). RESULTS: Diazinon or propafenone (1 × 10 -2 mol / L) significantly reduced the binding of GABA receptor (P <0.01), 1 × 10 -3 mol / L Of diazinon still significantly reduced the binding of GABA receptor (P <0.05). Diazinon 1.095 g / kg was intragastrically administered to rats. As a result, it was found that the binding value of 3 H-GABA was significantly decreased at the time of seizure onset. Rats were intraperitoneal injected with propafen 6 mg / kg to observe convulsive seizures and no seizures in rats 3 H-GABA binding values were significantly reduced. Conclusion: Organophosphorus pesticides can significantly reduce the binding of GABA receptor in rat brain at a certain concentration. GABA receptor is involved in the acute poisoning of organophosphorus pesticides