利用LB膜技术对表面压力—面积(π-A)曲线进行了测量及原子力显微镜(AFM)观测,研究了胆固醇对磷脂酰乙醇胺单分子层的影响.π-A曲线分析表明,随着胆固醇的增加,加速了相变的发生,促进膜稳定状态的形成.AFM观察结果表明,随着胆固醇比例的增加,混合膜呈现从点状结构向较大的片状与网状共存结构的转化,最终纯胆固醇形成高度紧密的膜结构. The effect of cholesterol on the monolayer of phosphatidylethanolamine was investigated by measuring the surface pressure-area (π-A) curve and atomic force microscopy (AFM) using the LB-membrane technique. The π-A curve analysis showed that with the increase of cholesterol Increase and accelerate the occurrence of phase transition and promote the formation of membrane stable state.AFM observations show that with the increase of cholesterol ratio, the hybrid membrane presents from the point-like structure to the larger sheet-like and network coexistence structure transformation, the final Pure cholesterol forms a highly dense membrane structure.