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用B_5培养基培养开花前油菜(Brassica napus)各期子房,发现各期子房均具高诱导率(98%以上).分化率也高,根为37.3%,芽为3.5%,由于外源激素浓度不同,子房出现多种形态.当2,4-D 为0.5毫克/升,BA 为0.2～0.5毫克/升时,子房壁特别膨大,当2,4-D为2毫克/升,BA 为0.2或1毫克/升时,子房一端形成愈伤组织,二者分化率不同,前者根的分化率高于后者,后者芽的分化率高于前者.又BA、NAA 混合使用较单独使用为好,其中以BA 为1～2毫克/升,NAA 为0.2毫克/升,根、芽分化率最高.
B5 medium was used to culture the ovary of Brassica napus and found high induction rate (98%) in each ovary, the rate of differentiation was also high, the root was 37.3% and the bud was 3.5% When the concentration of 2,4-D was 0.5 mg / L and the concentration of BA was 0.2 ~ 0.5 mg / L, the ovary wall was especially dilated. When 2,4-D was 2 mg / L, BA was 0.2 or 1 mg / L, the formation of calluses at one end of the ovary, the two different rates of differentiation, the former root differentiation rate higher than the latter, which latter bud differentiation rate higher than the former BA and NAA The mixed use is better than the single use, in which BA is 1 ~ 2 mg / L, NAA is 0.2 mg / L, the highest rate of root and shoot differentiation.