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目的探讨鲑鱼鱼白DNA(salmonmiltDNA,SMD)对小鼠胸腺增龄性萎缩的作用及作用机制。方法10月龄雌性BALB/c小鼠按体重随机分成缓冲液对照组(controlgroup,C组)、低剂量组(lowdosagegroup,L组)和高剂量组(highdosagegroup,H组),每组26只。在标准饲料基础上分别每日灌胃0.1mol/L的柠檬酸钠缓冲液和166.67mg/(kg·d),333.33mg/(kg·d)的SMD。5周后,无菌取胸腺,测量胸腺脏器指数;每个胸腺单独蜡块包埋,切片用Image-proPlus专业图像分析系统(4.0版)进行细胞计数和皮质厚度测量并经SAS(6.12版)统计软件分析数据。应用基因芯片技术在C组和H组胸腺组织中筛选表达差异的基因片段、RT-PCR鉴定部分片段。结果SMD对小鼠的体重、胸腺重量和胸腺指数均无影响(各指标F<3.0,P>0.05);高剂量组小鼠的胸腺皮、髓质细胞数量与对照组相比均显著增多犤皮质D(H,C)=9.46,P<0.01;髓质t(H,C)=2.53,P<0.05犦;低剂量组的胸腺皮、髓质细胞数量与对照组相比差异均不显著犤皮质D(L,C)=3.65,P>0.05;髓质t(L,C)=0.8,P>0.05犦;高、低剂量组的胸腺皮质平均厚度均显著高于对照组犤t(H,C)=4.01,P<0.01;t(L,C)=2.80,P<0.05犦;经基因芯片技术初筛出112条差异表达的基因片段;Genebank登录号为Aw209102,U23789,X80232的3条上调表达的基因片段,经RT-P
Objective To investigate the effect of salmonmilt DNA (SMD) on thymus-induced atrophy in mice and its mechanism. Methods 10-month-old female BALB / c mice were randomly divided into control group (group C), low-dose group (group L) and high-dose group (group H) with 26 rats in each group. On the basis of standard diet, 0.1mol / L sodium citrate buffer solution and 166.67mg / (kg · d), 333.33mg / (kg · d) SMD were administered daily. After 5 weeks, the thymus glands were aseptically measured, and the index of the thymus organs was measured. Each thymus was embedded in a separate wax block, and cell counts and cortical thickness measurements were performed with the Image-proPlus Professional Image Analysis System (version 4.0) Statistical software analyzes the data. Gene fragments were screened in C and H thymus tissues by gene chip technique, and some fragments were identified by RT-PCR. Results There was no significant effect of SMD on body weight, thymus weight and thymus index (F <3.0, P> 0.05). The number of thymus and medulla cells in high dose group increased significantly compared with the control group Cortical D (H, C) = 9.46, P <0.01; Medullary t (H, C) = 2.53, P <0.05 犦; There was no significant difference in the number of thymus skin and medulla cells between the low dose group and the control group (L, C) = 0.8, P> 0.05 犦. The mean thickness of thymus cortex in high and low dose groups was significantly higher than that in control group (P <0.05) 112 (p <0.01); t (L, C) = 2.80, P <0.05 犦; 112 differentially expressed genes were screened by gene chip technology; Genebank accession number Aw209102, U23789, X80232 Three up-regulated gene fragments, by RT-P