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目的 获取与半乳糖基抗CD3 单抗结合的肿瘤浸润淋巴细胞 (McAb TIL)杀伤自体肝癌细胞的形态学证据 ,并进行杀伤机制探讨。方法 制备McAb TIL ,将其与H2 2 小鼠肝癌细胞共同孵育 ,于不同时间在倒置显微镜和透射电镜下观察。结果 McAb TIL呈增殖活化状态 ,与靶细胞共同孵育 0 .5h即可导致靶细胞严重损伤。杀伤靶细胞后的McAb TIL结构清晰、完整。凋亡与坏死形态学改变可共存于同一个被杀伤靶细胞。结论 偶联了大量半乳糖基的抗CD3 单抗仍具有很强的肿瘤浸润淋巴细胞 (TIL)活化作用。McAb TIL通过多种杀伤机制杀伤自体肝癌细胞 ,杀伤力极强 ,具有连续杀伤靶细胞的能力。
Objective To obtain morphological evidence of killing hepatocarcinoma cells with McAb TIL conjugated with galactosyl anti-CD3 monoclonal antibody and investigate the mechanism of killing. Methods McAb TIL was prepared and incubated with H 2 2 hepatocarcinoma cells and observed under inverted microscope and transmission electron microscope at different times. Results McAb TIL was proliferative and activated state, and target cells co-incubated 0.5h can lead to serious damage to target cells. The McAb TIL structure after killing target cells is clear and complete. Morphological changes of apoptosis and necrosis can coexist in the same target cell. Conclusion The anti-CD3 monoclonal antibody coupled with a large amount of galactosyl still has a strong activation of tumor infiltrating lymphocytes (TILs). McAb TIL through a variety of killing mechanism to kill autologous liver cancer cells, highly lethal, with the ability to kill target cells in a row.