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青枯病是重庆番茄生产主要障碍之一,为选育抗病品种,在病理研究基础上,进行了抗源材料选育及利用研究。2000~2003年分离出番茄青枯病病菌68株,经致病性测定、培养性状、生化测定等研究,明确重庆市番茄青枯病病原菌为:Ralstoniasolanacearum,生理小种1,生化型Ⅲ。苗期室内人工接种抗性鉴定方法研究设伤根浸根接种法、灌注接种法、剪叶接种法三种处理,结果以伤根浸根接种法更为准确、可靠。经抗病性鉴定,结合农艺性状比较研究,筛选出四份抗源材料,并配组选出一个强优组合。
Bacterial wilt is one of the main obstacles in Chongqing tomato production. In order to breed resistant varieties, on the basis of pathological studies, the research on breeding and utilization of anti-source materials was carried out. A total of 68 tomato bacterial wilt pathogens were isolated from 2000 to 2003. The pathogenicity, biochemical characteristics and pathogenicity were used to determine the pathogen of tomato bacterial wilt in Chongqing: Ralstonia solanacearum, physiological races 1 and biochemical Ⅲ. Seedling indoor artificial inoculation resistance identification method wound root immersion root inoculation method, irrigation inoculation method, cut leaf inoculation three kinds of treatment, the results root wound immersion root inoculation method is more accurate and reliable. After identification of disease resistance and combining with agronomic traits comparative study, four anti-source materials were screened out and one strong combination was selected.