论文部分内容阅读
经过大量筛选，以雌性银杏叶片为外植体建立了生产黄酮的培养系统，并对细胞生长期、苯丙氨酸和放线菌素－Ｄ对银杏黄酮产量的影响做了研究，结果表明：银杏黄酮的积累在细胞指数生长期开始增加，于培养２５天时达到最高值，在此之前，先有ＰＡＬ活性的增加。培养１５天时，在培养基中加入０．１５ｇ·Ｌ－１的苯丙氨酸，１１天后收获，银杏总黄酮产量较对照增加了２２．３％，为原叶片含量的１．５倍；起始培养时加入０．３ｍｇ·Ｌ－１放线菌素－Ｄ，培养２６天后收获，银杏总黄酮量增加了８５．５％，为原叶片含量的２．６倍。
After extensive screening, the cultivation system of flavonoids was established by using female Ginkgo biloba leaves as explants. The effects of phenylalanine and actinomycin-D on the yield of ginkgo flavonoids were studied. The results showed that: Accumulation of ginkgetin began to increase during the exponential growth phase of the cell and reached its highest value at 25 days of culture, prior to the increase in PAL activity. After cultured for 15 days, 0.15g · L-1 phenylalanine was added into the medium and harvested after 11 days. The yield of total flavonoids in Ginkgo biloba increased by 22.3% and 1.5 times as that of the control At the beginning of culture, 0.3 mg · L -1 actinomycin-D was added, and after 26 days of cultivation, the total flavonoids of Ginkgo biloba increased by 85.5%, 2.6 times that of the original leaves.