论文部分内容阅读
本研究对202份大白菜自然群体材料进行简化基因组测序(Specific Locus Amplified Fragment Sequencing,SLAF),并通过测序深度、SLAF标签的多态性与参考基因组的相似程度等指标的筛选,获得了全基因组均匀分布的960个SLAF分子标记。结合相应材料的霜霉病病情调查结果,利用所述960个SLAF标记进行全基因组关联分析。结果显示:(1)在A01染色体上检测到1个新的与抗霜霉病关联的SLAF标记,即SLAFMarker A0124655323;(2)根据SLAFMarker A0124655323左右两翼最近标记之间的距离确定此热点区间为A01染色体24573724~24755150的物理范围;(3)利用该区间内的一个SNP开发出适用于KASP分型技术的SNP分子标记,在各亚群选择准确率均在80%以上;(4)在此区间找到抗病相关基因13个。本研究获得的新的抗病位点和开发的SNP分子标记,将有助于大白菜抗霜霉病分子育种。
In this study, 202 Locust Amplified Fragment Sequencing (SLAF) samples of natural populations of Chinese cabbage were obtained. Through sequencing, the polymorphism of SLAF tag and the similarity of reference genome were screened to obtain the whole genome 960 SLAF molecular markers evenly distributed. Combined with the results of the survey on the status of downy mildew in the corresponding materials, 960 SLAF markers were used for genome-wide association analysis. The results showed that: (1) A new SLAF marker associated with downy mildew was detected on A01 chromosome, SLAFMarker A0124655323; (2) According to the distance between the nearest markers of SLAFMarker A0124655323, the hotspot interval was A01 (3) Using SNPs in this interval to develop SNP markers suitable for KASP typing, the accuracy of each subgroup selection is over 80%; (4) In this interval Find 13 disease-related genes. The new disease-resistant loci and SNP markers developed in this study will help Chinese cabbage to resist downy mildew molecular breeding.