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目的研究两亲性酞菁锌光敏剂ZnPcS2P2介导的光动力疗法(ZnPc-PDT)联合GM-CSF、B7.1基因修饰的EL-4细胞,对小鼠淋巴瘤的抑制作用。方法应用反复多次转染的方法,分别将逆转录病毒pLXSN、pLXSNmGM-CSF、pLXS-NmB7.1导入EL-4细胞,分别命名为EL-4/pLXSN、EL-4/GM和EL-4/B7。进行ZnPc-PDT时,经荷瘤小鼠的尾静脉注射ZnPcS2P2,4h后局部用波长670nm的激光照射瘤体;光源为670nm的半导体激光仪。将40只荷瘤小鼠分为5组,每组8只,即对照组(不做任何处理)、EL-4/B7+EL-4/GM对照组、ZnPc-PDT对照组、ZnPc-PDT+EL-4/pLXSN对照组、ZnPc-PDT+EL-4/B7+EL-4/GM实验组。隔天测量瘤块的大小,观察荷瘤小鼠淋巴瘤的生长情况,并计算瘤块的相对体积(RTV)。观察荷瘤小鼠的生存期及瘤组织的病理学改变。结果实验组的生存率高于各个对照组(P<0.01)。Zn-Pc-PDT组小鼠的生存期略高于EL-4/B7+EL-4/GM组(P=0.01)。结论mB7.1、mGM-CSF基因修饰的瘤苗可显著增强ZnPc-PDT的抗肿瘤效应。
Objective To study the inhibitory effect of amphoteric ZnPcS2P2-mediated photodynamic therapy (ZnPc-PDT) combined with GM-CSF and B7.1 gene-modified EL-4 cells on mouse lymphoma. Methods The retroviral vectors pLXSN, pLXSNmGM-CSF and pLXS-NmB7.1 were transfected into EL-4 cells by repeated transfection, and named EL-4 / pLXSN, EL- 4 / GM and EL- 4 / B7. When ZnPc-PDT was performed, ZnPcS2P2 was injected into the tail vein of tumor-bearing mice for 4 and 4 hours, then the tumor was irradiated with a laser at a wavelength of 670 nm. The light source was a 670 nm semiconductor laser. The 40 tumor-bearing mice were divided into 5 groups with 8 mice in each group, namely control group (without any treatment), EL-4 / B7 + EL-4 / GM control group, ZnPc-PDT control group, ZnPc-PDT + EL-4 / pLXSN control group, ZnPc-PDT + EL-4 / B7 + EL-4 / GM experimental group. The size of tumor mass was measured every other day, the growth of tumor-bearing mouse lymphoma was observed, and the relative volume of tumor (RTV) was calculated. The survival of tumor-bearing mice and pathological changes of tumor tissue were observed. Results The survival rate of the experimental group was higher than that of each control group (P <0.01). The survival of mice in Zn-Pc-PDT group was slightly higher than that in EL-4 / B7 + EL-4 / GM group (P = 0.01). Conclusion The mB7.1, mGM-CSF gene modified tumor vaccine can significantly enhance the anti-tumor effect of ZnPc-PDT.