论文部分内容阅读
用放射配体受体结合分析法，观察了三氯乙醇（ＴＣＥｔ）对５－ＨＴ３受体激动剂５－ＨＴ和５－ＨＴ３受体拮抗剂ＩＣＳ２０５－９３０与［３Ｈ］ＢＲＬ１６１７０竞争大鼠脑皮质５－ＨＴ３受体能力的影响。结果证实，ＴＣＥｔ（４ｍｍｏｌ·Ｌ－１）能使５－ＨＴ竞争受体的浓度－反应曲线左移，半数抑制结合浓度降低（Ｐ＜０．０１），斜率无显著改变；而对ＩＣＳ２０５－９３Ｏ未见明显影响。表明ＴＣＥｔ能增强５－ＨＴ与５－ＨＴ３受体结合的能力，提示它可能通过这一机制促进５－ＨＴ３配体闸门离子通道开放而发挥作用。
Radioligand binding assay was used to observe the effects of trichloroethanol (TCEt) on rat brain cortex competition with 5-HT3 receptor agonist 5-HT and 5-HT3 receptor antagonist ICS205-930 and [3H] BRL16170 5-HT3 receptor capacity. The results showed that TCEt (4mmol·L-1) shifted the concentration-response curve of 5-HT competing receptor to the left and the half inhibitory concentration decreased (P <0.01), while the slope of ICS205-93O No obvious effect. The results showed that TCEt could enhance the binding of 5-HT to 5-HT3 receptor, suggesting that TCEt may play a role in promoting the opening of 5-HT3 ligand gate channel by this mechanism.