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目的研究变形链球菌表面蛋白PAc不同表达株在含蔗糖培养基中,及加氟至lppm或洗必太至0.005%的含蔗糖培养基中对玻壁表面粘附能力的差异。方法在厌氧培养条件下培养各株变形链球菌,用OD值测量法计算粘附量。结果在普通及加入氟至IPPm的含蔗糖培养基中,变链菌表面蛋白PAc不同表达株对玻壁的粘附无差异;而在含0.005%洗必太的培养基中,变形链球菌各菌株的粘附量显著下降。结论在变形链球菌大量合成葡聚糖的条件下,表面蛋白PAc在变形链球菌对玻壁粘附过程中未起决定作用。洗必太可能具有抑制葡聚糖合成的作用,而氟则没有。
Objective To study the differences in surface adhesion ability of Streptococcus mutans surface protein PAc in sucrose-containing medium and sucrose-containing medium with fluoride up to 1ppm or chlorhexidine up to 0.005%. Methods Streptococcus mutans were cultured under anaerobic conditions, and the amount of adhesion was calculated by OD value measurement. Results In the normal and added fluoride to IPPm containing sucrose medium, Streptococcus mutans surface protein PAc different strains of glass wall adhesion was no difference; in 0.005% chlorhexidine medium, the deformation of the chain The adhesion amount of each strain of cocci was significantly decreased. Conclusion In the case of Streptococcus mutans abundant dextran synthesis, the surface protein PAc in Streptococcus mutans adhesion to the glass wall is not a decisive role. Chlorhexidine may inhibit the synthesis of dextran, while fluoride does not.