论文部分内容阅读
用高效液相色谱法测定从(木奈)果实中纯化提取的3种植物内源激素GA_3、IAA.ABA(±),并介绍了植物组织中激素日常分析的提取和纯化过程。本实验以C_(18)柱为分离柱;甲醇:0.01mol/L,H_3PO_4(pH2.4)=42:58(v/v)为流动相,在流速1mL/min时恒流洗脱,λ=254nm处检测了3种内源激素。被测组分的含量与其峰面积有良好的线性关系(r=0.9996～1.000),加标回收率在92.13%～95.25%,同时文中还评估了不同贮藏温度和不同试剂处理对(木奈)果实中GA_3含量的影响。3种内源激素的最小检出限均在纳克级。
The endogenous phytohormones GA 3, IAA.ABA (±) of three kinds of plants extracted from the fruits of (Chennai) were determined by high performance liquid chromatography (HPLC). The extraction and purification of hormones from plant tissues were also introduced. In this experiment, the mobile phase consisted of C 18 column, methanol: 0.01mol / L, H 2 PO 4 (pH 2.4) = 42:58 (v / v) Three endogenous hormones were detected at 254 nm. (R = 0.9996-1.000), the spiked recoveries ranged from 92.13% to 95.25%. In the meantime, the effects of different storage temperature and different reagents on the activity of (Mina) were also evaluated. Effect of GA 3 content in fruit. The minimum detectable levels of the three endogenous hormones are in nanograms level.