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目的对河南省2010年手足口病监测标本进行病原分离及VP1基因测序,了解分离病毒的基因特征及分子流行病学特点。方法将2010年收集的手足口病患者粪便标本和肛拭子标本840份进行病毒分离鉴定并对34株病毒分离株测定肠道病毒71型VP1全序,利用生物信息学软件对序列分析,构建序列系统进化树。结果测序获得34株来自河南省11个地市的VP1全长序列,分离株间的VP1区核苷酸相似性为96.3%～100%,系统进化分析显示属于C4基因型的C4a亚群,所有分离株均处于同一进化分支,轻重症间无明显差别。在2003~2009年的河南省和其他7省亦发现有C4a亚群存在。结论 2010年河南EV71分离株为C4基因型的C4a亚群,河南省2008年以来的分离株与2004年以来的中国大陆优势株流行趋势完全一致。
Objective To isolate the pathogen and VP1 gene of hand, foot and mouth disease in Henan Province in 2010 and investigate the genetic characteristics and molecular epidemiological characteristics of the isolated virus. Methods Stool samples and 840 samples of anal swab collected in 2010 were isolated and identified by virus isolation. The total sequence of VP1 of enterovirus 71 was detected by 34 strains of viruses. Bioinformatics software was used to sequence and construct Sequence phylogenetic tree. Results The 34 full-length VP1 sequences of 11 isolates from Henan province were sequenced. The nucleotide similarity of VP1 between isolates was 96.3% -100%. Phylogenetic analysis showed that all the C4 subtypes belonged to C4 genotype Isolates were in the same branch of evolution, no significant difference between severe illness. C4a subpopulations were also found in Henan Province and other seven provinces in 2003-2009. Conclusion The EV71 isolates from Henan Province in 2010 were C4a subtypes of C4 genotype. The isolates since 2008 in Henan Province are completely consistent with the epidemic trend of dominant strains in mainland China since 2004.