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目的建立分子印迹固相萃取-分光光度法检测水样中孔雀石绿的方法。方法以孔雀石绿(MG)为模板分子,甲基丙烯酸(MAA)为功能单体,乙二醇二甲基丙烯酸酯(EGDMA)为交联剂,以本体聚合方法制备的分子印迹聚合物为填料自制固相萃取柱。对固相萃取的上样溶剂、干燥方法、淋洗溶剂等条件进行了优化。采用优化的条件对自来水和河水加标水样中的孔雀石绿进行萃取,再用分光光光度计检测。结果自来水和河水水样加标浓度在8～128μg/L范围内得到的回收率分别为86.3%～92.1%和83.5%～90.3%。结论本方法操作简单、灵敏度高,适用于环境水样中孔雀石绿的痕量检测。
Objective To establish a method for the determination of malachite green in water samples by molecularly imprinted solid phase extraction - spectrophotometry. Methods The malachite green (MG) as template molecule, methacrylic acid (MAA) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as crosslinking agent, the molecularly imprinted polymers Filler homemade solid-phase extraction column. The conditions of solid phase extraction solvent, drying method, elution solvent were optimized. The malachite green in tap water and river water samples was extracted with optimized conditions and then detected by spectrophotometer. Results The recoveries of tap water and river water spiked at 8 ~ 128 μg / L were 86.3% -92.1% and 83.5% -90.3%, respectively. Conclusion The method is simple, sensitive and suitable for trace detection of malachite green in environmental water samples.