Dense granule protein (GRA) is a secreted protein in Neospora caninum and Toxoplasma gondii,which plays an important role in forming parasitophorous vacuole (PV)[1-4].GRAs are thought to remodel and maintain the environment of the PV for intracellular survival and replication in T.gondii [5].The precise function of GRAs is still unknown [6-9].TgGRA14 protein is a new GRA protein that it is highly expressed and targeted to PV membrane and intravacuolar [5].However,the GRA14 of N.caninum (NcGRA14) has not been identified.