论文部分内容阅读
目的 验证以周围血淋巴细胞 (PBL)的微核率作为定量的生物照射剂量指标对监测体内照射性损害的实用性。方法 应用细胞因子阻滞的微核法测定人体部分放疗患者 (13例 )PBL中的微核率 ,以照射剂量测定法的数据表示不同范围的照射次数、肿瘤累积剂量、总积分剂量和等值总体吸收剂量。结果 这些患者的PBL中微核率随等值总体吸收剂量增加而增加 ,呈线性分布 ,并明显偏离普哇松分布 ;PBL的微核率随随访时间增加而普遍下降 ,下降率呈线性分布并与等值总体吸收剂量相关 (r =0 .7,p=0 .0 0 7) ,微核率的降低在不同个体有很大差异 ;9例患者在初次放疗后至少 4 8个月 ,PBL微核率高于相应的放疗前的水平 ,表明放疗诱导残留的细胞遗传学损害持续存在。结论 人类微核率为评价体内放射剂量提供一个可靠的生物学剂量指标 ;放疗结束后 ,升高的PBL微核率持续存在是放射诱导畸变细胞部分残留的一种反映
Objective To verify the usefulness of micronucleus rate of peripheral blood lymphocytes (PBLs) as a quantitative biological exposure dose indicator for the monitoring of radiation damage in vivo. Methods Micronuclei of cytokine blockade was used to determine the micronucleus rate in PBLs of patients with partial radiotherapy (13 cases). The data of irradiation dosimetry were used to show the numbers of irradiation, tumor cumulative dose, total dose and equivalence Total absorbed dose. Results The micronucleus rate of PBL in these patients increased linearly with the increase of the equivalent total absorbed dose and obviously deviated from the distribution of Pwavon. The micronucleus rate of PBL decreased generally with the increase of follow-up time, and the rate of decrease was linear The decrease of micronucleus rate was significantly different in different individuals with equivalent total absorbed dose (r = 0.7, p = 0.0007). In 9 patients at least 48 months after initial radiotherapy, PBL The micronucleus rate is higher than the corresponding pre-radiotherapy level, indicating that radiation-induced residual cytogenetic damage persists. Conclusion The human micronucleus rate provides a reliable biological dose index for the evaluation of in vivo radiation dose. The persistence of micronucleus rate in PBL after radiotherapy is a reflection of the residual radioactivity-induced aberrant cells