论文部分内容阅读
80年代中期建立的聚合酶链反应(PCR)技术,以基因组DNA为模板在DNA聚合酶催化作用下,由一对寡核苷酸引物引导,在体外扩增靶DNA片段。经过约30个循环反应,靶DNA片段可扩增100万个拷贝。PCR技术使法医物证检验的灵敏度大为提高,达到纳克级DNA的超微量水平。近10年来,PCR技术在法医界迅速得以推广与使用,被称为第二代DNA分型技术。人类基因组内可变数目串联重复序列(VNTR)位点具有极高多态性,VNTR位点和片段长度等位基因多达数十,甚至数百。运用PCR技术扩增VNTR位点并经片段长度多态
Polymerase chain reaction (PCR) technology, established in the mid-1980s, uses genomic DNA as a template under the action of DNA polymerase and is guided by a pair of oligonucleotide primers to amplify the target DNA fragment in vitro. After about 30 cycles of reaction, the target DNA fragment can amplify 1 million copies. PCR technology so that forensic evidence test sensitivity greatly improved to reach nanograms level of ultra-trace DNA. The past 10 years, PCR technology in the forensic community to quickly promote and use, known as the second generation of DNA typing technology. The VNTR locus in the human genome is highly polymorphic, with as many as 10 or even hundreds of alleles per VNTR locus and fragment length. VNTR sites were amplified by PCR and subjected to fragment length polymorphism