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提取发病植株叶片及茎髓组织DNA,用玉米丝黑穗病菌特异引物进行PCR检测,验证叶片DNA对玉米丝黑穗病研究的可靠性。结果发现,提取叶片DNA研究玉米丝黑穗病的可靠性远低于茎髓组织DNA。
The DNA of diseased plant leaves and stem tissues was extracted, and the PCR was carried out by using the primers specific to the disease, to verify the reliability of leaf DNA on maize head smut. The results showed that the reliability of extracting leaf DNA for maize head smut was much lower than that of the stalk DNA.