论文部分内容阅读
动物的神经中枢在发育过程中存在神经元的自发死亡现象。例如,在鸡胚发育过程中,其脊髓腰部前角运动细胞外侧柱的细胞数目从胚胎第6天的22838±731个减至胚胎第10天的15018±537个,减少了34%。神经元的死亡或存活与靶组织等许多因素有关。在神经肌肉接头形成过程中,脊髓内cGMP含量增加的现象,引起了人们的兴趣。作者选用5～9天胚龄的鸡胚,每天向已形成血管的绒毛膜尿囊上滴加0.91mg/0.2ml的二丁酰cGMP。在胚龄第10天处死胚胎,经过固定、包埋、染色,连续切片等步骤,计算脊髓腰部外侧柱的细胞数,共19540±516个,仅比第6天下降14%,说明二丁酰cGMP可下降细胞的自发死亡率。用等克分子的8-溴cGMP处理鸡胚,作用大致相同。二丁酰cGMP降低神经元自发死亡率可能是
The nerve center of an animal has the phenomenon of spontaneous death of neurons during development. For example, during the development of chicken embryos, the number of extracellular lateral column cells in the anterior horn of the spinal cord decreased from 22838 ± 731 on the 6th day of embryos to 15018 ± 537 on the 10th day of embryos, a 34% decrease. Neuronal death or survival depends on many factors such as the target tissue. During the process of neuromuscular junction formation, the increase of cGMP content in the spinal cord has aroused people’s interest. The authors selected embryos of 5-9 days embryonic age embryos, to the vascularized chorioallantoic drip daily added 0.91mg / 0.2ml of dibutyryl cGMP. The embryos were sacrificed on day 10 of embryo age, and fixed, embedded, stained and serial sections were used to calculate the number of cells in the lateral column of the spinal cord, which was 19540 ± 516, only 14% less than that on the 6th day. cGMP can reduce the spontaneous mortality of cells. Chicken embryos were treated with an equal molecular weight 8-bromo cGMP for approximately the same effect. Dibutyryl cGMP reduces neuronal spontaneous mortality may be