论文部分内容阅读
目的:观察大黄对洛哌丁胺便秘模型大鼠肠道动力以及结肠AQP3功能和表达的干预作用,探讨大黄发挥通便效应的作用机制。方法:50只Wistar大鼠随机分为5组,分别是空白组(A)、模型组(B)、大黄+消炎痛组(C)、大黄+安慰剂组(D)、消炎痛+安慰剂组(E)。采用洛哌丁胺(1.5mg·kg~(-1)·d~(-1))给予B、C、D、E组大鼠灌胃7 d,建立便秘模型,处死A、B组大鼠比较一般情况、粪便含水量、炭末推进率以验证模型。随后,用生大黄粉(5 g·kg~(-1))混悬液予C、D组大鼠灌胃,E组则采用同等剂量生理盐水。C、E组大鼠灌胃前以消炎痛(10mg·kg~(-1))预处理,D组则使用生理盐水代替。5 h后处死C、D、E组大鼠。所有大鼠处死前均以10%活性炭悬液灌胃,测定炭末推进率。采用RT-PCR、免疫组织化学法、ELISA检测大鼠近端结肠组织中AQP3表达情况以及PGE2、VIP的活性水平。结果:1造模后大鼠的体质量增加、结肠存留粪便增多、粪便含水量降低、肠道传输时间延长(均P<0.05)。2大黄干预便秘大鼠,可加快肠道传输、增加粪便含水量、降低VIP浓度、升高PGE2含量、下调AQP3 mRNA及蛋白表达水平(均P<0.05)。3消炎痛能够显著抑制大黄的通便效应,减少粪便含水量、降低PGE2浓度、上调AQP3 mRNA及蛋白表达水平(均P<0.05),但对肠道传输和VIP无明显影响(均P>0.05)。结论:1通过刺激结肠产生PGE2,快速下调上皮细胞质膜AQP3表达水平,抑制结肠吸收水分,可能是大黄治疗便秘的作用机制之一。2大黄可增强便秘大鼠的肠传输功能,可能与其降低结肠VIP水平,促进肠道蠕动有关。
OBJECTIVE: To observe the effect of rhubarb on intestinal motility and the function and expression of colon AQP3 in loperamide constipation model rats, and to explore the mechanism of action of rhubarb on catharsis. Methods: Fifty Wistar rats were randomly divided into five groups: blank group (A), model group (B), rhubarb plus indomethacin group (C), rhubarb + placebo group (D), indomethacin + placebo Group (E). Rats in groups B, C, D and E were treated with loperamide (1.5 mg · kg -1 d -1) for 7 days, and constipation models were established. Rats in groups A and B were sacrificed Compare the general situation, stool water content, carbon powder propulsion rate to validate the model. Subsequently, the rats were inoculated with the suspension of rhubarb powder (5 g · kg ~ (-1)) into groups C and D, and the rats in group E received the same dose of normal saline. Rats in groups C and E were pretreated with indomethacin (10 mg · kg -1) before administration and saline (saline) instead of group D. After 5 h, rats in groups C, D and E were sacrificed. All rats were sacrificed by 10% activated carbon suspension before gavage, determination of carbon propulsion rate. The expression of AQP3 and the activity of PGE2 and VIP in the proximal colon of rats were detected by RT-PCR, immunohistochemistry and ELISA. Results: After the model was established, the body weight of the rats increased, the stool in the colon increased, the water content in the stool decreased and the intestinal transit time prolonged (all P <0.05). 2 rhubarb intervention constipation rats, can accelerate the intestinal tract transport, increased water content of feces, reduce VIP concentration, increased PGE2 content, down AQP3 mRNA and protein expression levels (all P <0.05). 3 indomethacin significantly inhibited rhubarb’s laxative effect, decreased stool water content, reduced PGE2 concentration and up-regulated AQP3 mRNA and protein expression (all P <0.05), but had no significant effect on intestinal transit and VIP (all P> 0.05 ). Conclusion: 1 by stimulating the colon produce PGE2, rapid down-regulation of plasma membrane AQP3 expression in epithelial cells, inhibition of colon absorption of water may be one of the mechanisms of rhubarb in the treatment of constipation. 2 Rhubarb can enhance the intestinal transit function of constipation rats may be related to its lower VIP level of colon and promote intestinal peristalsis.