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1973年DeGast等及1974年本文作者等首先提出急、慢性乙型肝炎的病损,根据其形态病理学的特征,可能由于淋巴细胞对HBsAg所产生的免疫反应而引起,即所谓细胞介导(Cell mediated)性免疫反应所引起。随后许多学者发表文章也支持了此点,但实验证明的主要困难就是难于建立一个实验性培养条件的靶细胞。本研究就是为此目的建立一种实验性靶细胞为手段,来进行急、慢性肝炎病人的细胞介导性免疫反应。靶细胞的制备方法如下:以张氏(Chang细胞0.1ml内含10~6个细胞,加入0.2ml内含6.0μg/ml的HBsAg,用bisdiazotized benzidine(BDB)试剂为联结剂,置室温30
DeGast et al., 1973 and 1974 The author first proposed acute and chronic hepatitis B lesions, which may be due to the immunological reaction of lymphocytes to HBsAg according to their morphological and pathological features. The so-called cell-mediated Cell mediated immune response caused. This is supported by many articles published by many scholars later, but the main difficulty proved by experiments is that it is difficult to establish target cells for experimental culture conditions. The purpose of this study is to establish an experimental target cells as a means to carry out cell-mediated immune response in patients with acute and chronic hepatitis. The target cells were prepared as follows: 0.1 ml of Chang cells containing 10 to 6 cells, 0.2 ml of HBsAg containing 6.0 μg / ml, bisdiazotized benzidine (BDB) reagent, and room temperature 30