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目的通过检测并比较肿瘤坏死因子样凋亡弱诱导剂(TWEAK)和人成纤维细胞生长因子诱导型14(Fn14)在正常胰腺组织、慢性胰腺炎及胰腺癌组织中的表达,分析探讨TWEAK/Fn14通路在胰腺癌发展过程中的作用。方法收集在四川大学华西医院胰腺外科行手术治疗、切除的胰腺组织标本,并经病理检察证实,胰腺癌标本50例,慢性胰腺炎标本40例,正常胰腺组织标本30例。采用免疫组化检测胰腺组织中TWEAK和Fn14的表达,并分析TWEAK表达与胰腺癌临床病理特征的关系。结果TWEAK阳性表达率在胰腺癌为36.0%(18/50),高于慢性胰腺炎17.5%(7/40)及正常胰腺组织13.3%(4/30),组间差异有统计学意义(P<0.05),表达强度亦为胰腺癌组织>慢性胰腺炎>正常胰腺组织(P<0.05)。Fn14在胰腺癌组织(4.0%)和正常胰腺组织(7.0%)阳性表达率低,胰腺炎组织中未发现阳性表达。TWEAK阳性表达率和高表达率在Ⅱ期患者中随着肿瘤疾病分级增加而增加(P<0.05)。而各病理分级中,TWEAK阳性表达率、高表达率和EI评分的差异均无统计学意义。结论 TWEAK/Fn14参与了胰腺癌的发生发展过程。TWEAK在胰腺癌中高表达,Fn14呈低表达。
Objective To detect and compare the expressions of TWEAK and Fn14 in normal pancreatic tissue, chronic pancreatitis and pancreatic cancer tissues, and to explore the role of TWEAK / The Role of Fn14 Pathway in the Development of Pancreatic Cancer. Methods Pancreatic tissue specimens were collected from the Department of Pancreatic Surgery, West China Hospital of Sichuan University. Pathological examination confirmed 50 cases of pancreatic cancer, 40 cases of chronic pancreatitis and 30 cases of normal pancreatic tissue. Immunohistochemistry was used to detect the expression of TWEAK and Fn14 in pancreatic tissues, and the relationship between TWEAK expression and the clinicopathological features of pancreatic cancer was analyzed. Results The positive rate of TWEAK was 36.0% (18/50) in pancreatic cancer, higher than 17.5% (7/40) in chronic pancreatitis and 13.3% (4/30) in normal pancreatic tissue, the difference was statistically significant (P <0.05). The expression intensity was also pancreatic cancer> chronic pancreatitis> normal pancreatic tissue (P <0.05). The positive expression rate of Fn14 in pancreatic cancer tissues (4.0%) and normal pancreatic tissues (7.0%) was low, and no positive expression was found in pancreatic tissues. The positive rate and high expression rate of TWEAK increased with the increase of tumor grade in stage Ⅱ patients (P <0.05). However, there was no significant difference in the expression of TWEAK, the high expression rate and the EI score among the pathological grades. Conclusion TWEAK / Fn14 is involved in the development of pancreatic cancer. TWEAK is highly expressed in pancreatic cancer, Fn14 is low expression.