论文部分内容阅读
目的 定量观察胶质细胞源性神经营养因子(glial cell ine-derived neurotrophic factor,GDNF)和神经营养-3(neurotrophin-3,NT-3)对噪声引起豚鼠耳蜗外毛细胞损伤的防护作用。方法 将微渗透压泵埋置于豚鼠背部,经固定于耳蜗底回鼓阶内的改良微导管将GDNF(100ng/ml)和NT-3(2.5μg/ml)的混合液缓慢注入12只豚鼠左侧内耳,以左侧内耳灌注人工外淋巴液的9只豚鼠为对照,检测噪声暴露后豚鼠听功能和耳蜗外毛细胞形态、数量的变化。结果 噪声暴露10天后,实验组手术耳和非手术耳的脑干诱发电反应阈值低于对照组(P<0.05,p<0.01)。毛细胞表皮板和纤毛肌动蛋白荧光染色计数发现,实验组手术耳和非手术耳的外毛细胞缺失率低于对照组(p<0.001,p<0.01)。毛细胞核荧光染色计数发现,实验组手术耳和非手术耳的外毛细胞核肿胀率低于对照组(p<0.01,p<0.01)。结论 GDNF和NT-3对噪声引起豚鼠耳蜗外毛细胞损伤具有较好的防护作用。
Objective To quantitatively observe the protective effect of glial cell-derived neurotrophic factor (GDNF) and neurotrophin-3 (NT-3) on noise-induced cochlear outer hair cell injury in guinea pigs. Methods The osmotic pump was embedded in the back of guinea pig and the mixture of GDNF (100 ng / ml) and NT-3 (2.5 μg / ml) was slowly injected into 12 guinea pigs The left inner ear was treated with 9 guinea pigs that were perfused with artificial left lymph on the left internal ear. The changes of auditory function and cochlear outer hair cell morphology and number were examined after noise exposure. Results After 10 days of noise exposure, the threshold of brainstem response in the experimental and non-surgical ears was lower than that of the control group (P <0.05, p <0.01). The counts of hair cell epidermis and cilia actin in the experimental group were lower than those in the control group (p <0.001, p <0.01). Fluorescent staining of hair cell nuclei showed that the rate of outer hair cell nuclear edema in the experimental and non-surgical ears was lower than that in the control group (p <0.01, p <0.01). Conclusion GDNF and NT-3 have a good protective effect against noise-induced cochlear outer hair cell injury in guinea pigs.