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一步探讨西比灵对急性脑缺血再灌注损伤的保护作用机制。方法 采用Pulsinelli四血管闭塞法制备大鼠急性脑缺血再灌注模型 ,利用Tsuchida的WSPS法测定LPO ,利用化学发光法测定SOD活性 ,同时观察了脑组织水含量及病理变化 ,并观察西比灵对上述指标影响。结果 ①脑缺血后脑组织水含量升高 ,再灌注后升高更明显 ,西比灵能明显降低脑缺血再灌注期间脑水含量。②西比灵降低LPO含量 ,稳定SOD活性。③图像分析显示西比灵可增加再灌注后神经元面数密度。结论 西比灵不但可防止Ca2 +过量跨膜进入细胞内 ,还具有抑制血由基生成的作用 ,减轻脑水肿 ,减轻脑组织的病理损害 ,从而在脑缺血再灌注期间达到脑保护作用
A step-by-step study on the protective effect of xibeiling on acute cerebral ischemia-reperfusion injury. Methods A rat model of acute cerebral ischemia-reperfusion was established by Pulsinelli four-vessel occlusion method. LPO was determined by WSPS method of Tsuchida. The activity of SOD was measured by chemiluminescence method. The water content and pathological changes of brain tissue were also observed. Impact on the above indicators. Results ① After cerebral ischemia, the water content of brain tissue increased and became more obvious after reperfusion, and Sibelium significantly reduced the water content of brain during cerebral ischemia-reperfusion. ② Xi Bilin lower LPO content, stable SOD activity. ③ Image analysis showed that Sibelium increased the density of neurons after reperfusion. Conclusion Xibeilin can not only prevent Ca2 + from transmembrane entry into the cells, but also inhibit the generation of blood from the base, reduce cerebral edema, alleviate the pathological damage of brain tissue and thus achieve cerebral protection during cerebral ischemia-reperfusion