目的建立雷公藤内酯醇生物贴的质量标准,雷公藤内酯醇生物贴中雷公藤内酯醇的定量测定方法。方法雷公藤内酯醇生物贴经甲醇超声提取。色谱柱为Agilent Zorbax SB-C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-水(3∶7),检测波长为220 nm。结果雷公藤内酯醇在5~120μg/mL,线性关系良好(r=0.9981)。平均回收率96.5%(n=9)。RSD<3%。结论本方法重现性好,灵敏度高。 Objective To establish the quality standard of triptolide bio-paste and the quantitative determination of triptolide in triptolide bio-paste. Methods The triptolide alcohol was extracted by ultrasonic extraction with methanol. The column was an Agilent Zorbax SB-C18 column (250 mm×4.6 mm, 5 μm) with a mobile phase of acetonitrile-water (3:7) and a detection wavelength of 220 nm. The results showed that the triptolide had a good linearity (r=0.9981) at 5~120μg/mL. The average recovery rate was 96.5% (n=9). RSD < 3%. Conclusion This method has good reproducibility and high sensitivity.