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目的利用ｍＲＮＡ差异显示技术分析在遗传不稳定性产生和传递过程中，是否有一些特异的基因表达的变化，并在此基础上了解哪些基因在此过程中起关键作用。方法人胚胎成纤维细胞ＷＩ３８经γ射线０５、１０、３０、５０、７０Ｇｙ照射后８小时及３Ｇｙ照射后４、８、２４、４８、１２０小时，提取细胞ＲＮＡ，进行ｍＲＮＡＤＤＰＣＲ，并将差异表达片段进行再扩增。结果ＰＣＲ扩增产物经电泳后，有１４条表达差异的带型出现，所有差异表达片段均获得了再扩增。结论结果说明，某些特异的基因变化参与到了这一过程中，根据我们以前的实验推测，很可能是修复基因的改变。目前正在对这些未知片段进行克隆和序列分析。
OBJECTIVE: To use mRNA differential display to analyze whether there are some specific changes in gene expression in the process of genetic instability generation and transmission, and on the basis of which genes play a key role in this process. Methods Human embryonic fibroblasts WI 38 after γ rays 0 5,1 0,3 0,5 0,7 0Gy 8 hours after irradiation and 3Gy irradiation 4,8,24,48,120 hours, Cell RNA was extracted and subjected to mRNA-DPCR, and the differentially expressed fragments were re-amplified. Results After the PCR products were electrophoresed, 14 bands with different expression appeared and all the differentially expressed fragments were re-amplified. Conclusion The results suggest that some specific genetic changes are involved in this process, which, according to our previous experiments, is likely to be a modification of the repair gene. These unknown fragments are currently being cloned and sequenced.