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目的探讨子宫肌瘤组织中血小板衍生因子(PDGF)和真核起始因子4E(eIF4E)的表达情况及其临床意义。方法选取2013年4月-2016年10月该院收治的子宫肌瘤患者86例,行子宫切除术后,立即获取子宫肌瘤组织;另外,在至少距离肌瘤组织2 cm处获取周围正常组织67例。采用SP染色法检测标本中PDGF和eIF4E表达水平,分析PDGF和eIF4E表达情况与子宫肌瘤间的关系,并分析PDGF与eIF4E的相关性。结果 PDGF可以在子宫肌瘤组织和周围的正常组织中表达,主要集中在细胞质或细胞核中;eIF4E主要表达在子宫肌瘤组织的细胞质中;子宫肌瘤组织中PDGF和eIF4E的阳性表达率分别为80.23%(69/86)和83.72%(72/86),均明显高于周围正常组织的11.94%(8/67)和4.48%(3/67),差异均有统计学意义(均P<0.05);除子宫肌瘤的不同时期外,PDGF和eIF4E的阳性表达率与患者的年龄、肌瘤大小和肌瘤个数存在明显的相关性(P<0.05);PDGF和eIF4E在正常组织中的表达无相关性(r=0.324,P=0.395>0.05),而在子宫肌瘤组织中的表达呈正相关(r=0.765,P=0.016<0.05)。结论 PDGF和eIF4E在子宫肌瘤组织中高表达,并且与子宫肌瘤的相关参数具有密切联系,临床上可用于预测和诊断子宫肌瘤的发生与发展。另外两者在子宫肌瘤组织中的表达呈正相关,为临床上研发治疗子宫肌瘤的药物提供新的靶点。
Objective To investigate the expression of platelet-derived factor (PDGF) and eIF4E in uterine fibroids and its clinical significance. Methods From April 2013 to October 2016, 86 cases of uterine fibroids were treated in our hospital. Uterine fibroids were obtained immediately after hysterectomy. In addition, the normal tissues around 2 cm away from myoma were obtained 67 cases. SP staining was used to detect the expression of PDGF and eIF4E, the relationship between PDGF and eIF4E expression and uterine myoma were analyzed, and the correlation between PDGF and eIF4E was analyzed. Results PDGF could be expressed in uterine fibroids and surrounding normal tissues, mainly in the cytoplasm or nucleus; eIF4E was mainly expressed in the cytoplasm of uterine fibroids; the positive rates of PDGF and eIF4E in uterine fibroids were 80.23% (69/86) and 83.72% (72/86), respectively, which were significantly higher than that of the surrounding normal tissues (11.94% and 4.48%, 3/67 respectively) (all P < 0.05). The positive expression rates of PDGF and eIF4E were significantly correlated with the age, fibroid size and the number of myoma (P <0.05) except for the different stages of uterine fibroids. The expressions of PDGF and eIF4E in normal tissues (R = 0.324, P = 0.395> 0.05), while there was a positive correlation in the expression of uterine myoma (r = 0.765, P = 0.016 <0.05). Conclusion PDGF and eIF4E are highly expressed in uterine fibroids and closely related to the related parameters of uterine fibroids. They can be used clinically to predict and diagnose the occurrence and development of uterine fibroids. The other two showed a positive correlation in the expression of uterine fibroids, which provided a new target for the clinical research and development of drugs for treating uterine fibroids.