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目的:探讨盐酸苯肼注射浓度对小鼠血虚状态的影响,改良小鼠化学损伤性血虚证模型。方法:将ICR小鼠随机分为空白对照组(不进行造模)和4个试验组;试验组分别于第1、4、7天腹腔注射不同浓度的盐酸苯肼,检测造模前后小鼠体重、外周血红细胞数目和血红蛋白含量的变化,分析血虚状态持续时间。结果:腹腔注射盐酸苯肼可使ICR小鼠的外周血红细胞数量和血红蛋白含量显著降低(P<0.01),出现明显的中医血虚证表现。这一效应存在量效关系,但高浓度的盐酸苯肼影响小鼠存活率。结论:盐酸苯肼介导小鼠血虚证模型的最佳给药浓度为60 mg·kg-1体重。该模型的血虚状态至少可持续7 d,有利于进行相关科学研究。
Objective: To investigate the effects of phenylhydrazine hydrochloride injection on blood deficiency status in mice and to improve the model of chemical-induced blood deficiency syndrome in mice. Methods: The ICR mice were randomly divided into blank control group (no modeling) and 4 experimental groups. The experimental groups were injected intraperitoneally with different concentrations of Phenylhydrazine hydrochloride on day 1, Weight, peripheral blood red blood cell count and hemoglobin content changes, analysis of blood deficiency state duration. Results: Phenylhydrazine hydrochloride intraperitoneally induced peripheral blood red blood cell count and hemoglobin in ICR mice significantly decreased (P <0.01), and obvious blood deficiency syndrome of TCM appeared. There is a dose-effect relationship between this effect, but high concentrations of phenylhydrazine hydrochloride affect the survival rate of mice. CONCLUSION: The optimal concentration of phenylhydrazine hydrochloride-induced blood deficiency syndrome in mice is 60 mg · kg-1 body weight. The blood deficiency status of the model lasts for at least 7 days, which is conducive to related scientific research.