论文部分内容阅读
在疟疾混合感染的流行区,恶性疟与间日疟的混合感染常不易被镜检所发现,以致漏诊,影响治疗。作者用聚合酶链反应(PCR)技术检测从疟区采集到的疟疾病人血样,同时与常规镜检的结果进行了比较,检出了较多的混合感染病人。检测对象为驻泰国疟疾流行区军人中的疟疾病人。镜检结果是由有经验的镜检员油镜下检查吉姆萨染色的厚、薄血片所得,同时末梢取血25μl,一式三份滴加于Whatman3~#滤纸,自然晾干后装塑料袋中室温保存。干燥滤纸血样经Chelex-100处理后,吸取
In endemic areas with mixed malaria infection, the mixed infection of P. falciparum and P. vivax is often not easily detected by microscopic examination, resulting in missed diagnosis and treatment. The authors used polymerase chain reaction (PCR) to detect blood samples from malaria patients collected from malaria patients, and compared with the results of routine microscopy, detecting more patients with mixed infections. The test subjects were malaria patients among the malaria-endemic areas in Thailand. Microscopic examination results by an experienced mirror inspector under the auspicious Giemsa staining of thick, thin blood film obtained, while the peripheral blood 25μl, in triplicate drops of Whatman3 ~ # filter paper, dried in a plastic bag Store at room temperature. Dry filter paper samples after Chelex-100 treatment, drawing