论文部分内容阅读
Alam等(1969)以苏丹草花药为材料,进行正极向过氧化物酶同工酶电泳分析,结果表明;雄性不育系的酶带数少于保持系。而代尧仁等(1978)在水稻,及傅鸿仪等(1980)在高梁花药中分别得出与Alam等相反的结果。我们以籼稻野败型雄性不育系、保持系、恢复系以及F_1幼苗进行了负极向和正极向聚丙烯酰胺凝胶电泳分析,发现当进行正极向电泳时,其酶带数不育系少于保持系,恰与Alam的结果相似(待发表);而当进行负极向电泳时,则情况相反,不育系酶带数明显地多于保持系。这种雄性不育系与保持系之间存在的过氧化物酶同工酶谱的差异,可能是由于细胞质雄性不育基因表达的调控所造成的。
Alam et al. (1969) used Sudan grass anther as a material to conduct positive electrode analysis of peroxidase isozymes. The results showed that the number of bands of male sterile lines was less than that of maintainer lines. On the other hand, Yao et al. (1978) obtained the opposite results from Alam in rice and Fu Hongyi et al. (1980) in sorghum anthers respectively. We carried out the negative and positive polyacrylamide gel electrophoresis analysis on male sterile lines, maintainer lines, restorer lines and F_1 seedlings of indica wild-type male sterile lines, and found that when positive polarity electrophoresis was carried out, the number of male sterile lines In the maintainer line, the result is similar to that of Alam (to be published), whereas in the case of negative polarity electrophoresis, the opposite is true, with significantly more bands in the sterile line than in the maintainer line. The difference in the peroxidase isoenzyme pattern existing between this male sterile line and the maintainer line may be due to the regulation of cytoplasmic male sterility gene expression.