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目的 探讨 4 氨基吡啶 (4 AP)对小细胞肺癌 (SCLC)中电压激活性K+ 电流和细胞增殖的抑制作用。方法 运用全细胞膜片钳技术 ,记录和分析 4 AP对SCLC中电压激活性K+ 电流的抑制作用 ;运用流式细胞仪 ,检测 4 AP对SCLC细胞增殖周期的影响 ;通过细胞体外增殖试验 ,证实 4 AP对SCLC细胞增殖的抑制作用。结果 5mmol/L的 4 AP可将峰值外向K+ 电流 (除极化到 +80mV时激活 )从 1.2 2± 0 .11nA降低到 0 .5 9± 0 .10nA。流式细胞仪检测结果显示 ,应用 4 AP 3d后 ,能使SCLC细胞增殖周期中的G0 /G1期阻滞 ,S期和G1/M期的比率下降 (P <0 .0 1) ;0 .1,5 ,10 ,15 ,2 0mmol/L的 4 AP均能抑制SCLC细胞体外增殖 ,抑制的程度与药物的浓度、作用时间有关。结论 电压激活性K+ 通道在SCLC细胞增殖过程中起重要作用 ,K+ 通道阻滞剂能抑制SCLC细胞的增殖。
Objective To investigate the inhibitory effect of 4-aminopyridine (4 AP) on voltage-activated K + current and cell proliferation in small cell lung cancer (SCLC). Methods Whole-cell patch-clamp technique was used to record and analyze the inhibitory effect of 4 AP on voltage-activated K + current in SCLC. Flow Cytometry was used to detect the effect of 4 AP on the proliferation of SCLC cells. The in vitro proliferation assay confirmed that 4 Inhibition of AP on proliferation of SCLC cells. Results 5 mmol / L 4 AP reduced peak extrinsic K + current (depolarization to +80 mV activation) from 1.2 2 ± 0.1 1 nA to 0 .59 ± 0 .10 nA. Flow Cytometry showed that the cell cycle of G0 / G1 arrest, the ratio of S phase and G1 / M phase decreased (P <0.01) after 4 days of AP3d; 1, 4, 5, 10, 15, 20mmol / L 4 AP can inhibit SCLC cell proliferation in vitro, the degree of inhibition and drug concentration, duration of action. Conclusion Voltage-activated K + channels play an important role in the proliferation of SCLC cells. K + channel blockers can inhibit the proliferation of SCLC cells.