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人外周血淋巴细胞,在含Ca~(2+)、Mg~(2+)的Hanks液中与绵羊红细胞(SRBC)共育,能将SRBC破坏释出血红蛋白(Hb),半数溶血值为0.1921±0.06,经胰蛋白酶抑制后淋巴细胞破坏SRBC的能力明显减弱,半数溶血值为0.0348±0.03。经尼龙棉柱法及E-玫瑰花法把淋巴细胞分离为T、B和NK等各个亚群。引起溶血作用最强的是NK细胞。对同一血源标本,同时用~(125)IUdR释放法测定NK活性和溶血分光光度法测定溶血活性,其结果呈明显正相关。本文认为人外周血淋巴细胞有破坏绵羊红细胞的能力,有这种能力的淋巴细胞是NK细胞。可望用本实验的溶血分光光度法测定NK细胞的活性。
Human peripheral blood lymphocytes, co-cultured with sheep erythrocytes (SRBC) in Hanks fluid containing Ca 2+ and Mg 2+ , can destroy SRBC hemoglobin hemoglobin (Hb), with a half hemolysis value of 0.1921 ± 0.06, the ability of lymphocytes to destroy SRBC after trypsin inhibition was significantly weakened, half of the hemolytic value was 0.0348 ± 0.03. The lymphocytes were separated into T, B and NK subgroups by nylon cotton column method and E-rose method. The strongest hemolytic effect is NK cells. For the same blood specimens, NK activity was measured by ~(125)IUdR release method, and hemolytic activity was measured by hemolytic spectrophotometry. The results were positively correlated. This article believes that human peripheral blood lymphocytes have the ability to destroy red blood cells in sheep. The lymphocytes with this ability are NK cells. It is expected that the activity of NK cells can be measured by the hemolytic spectrophotometry of this experiment.