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应用石蜡切片和生理生化测定等方法,对15种松树最易遭受枯梢病菌侵染的嫩梢,进行形态结构解剖学检测和皮层内苯丙氨酸解氨酶(PAL)活性测定,探讨其抗病机制。研究结果表明,在形态结构解剖学方面,抗病和感病类型差别,与周皮组织结构有关,与皮层组织结构无关,前者抗病类型周皮细胞多为2~4层,周皮组织厚36.0-100.8um;。高度感病类型——周皮细胞多为1层,周皮组织厚14.4-24.0um。在生理生化方面,抗病类型苯丙氨酸解氨酶活性均高于感病类型。
The morphological and anatomical characteristics of the shoots and the phenylalanine ammonia-lyase (PAL) activity in the cortex of 15 kinds of pine trees were studied by using paraffin section and physiological and biochemical methods. Disease-resistant mechanism. The results showed that in the morphological anatomy, the difference of disease resistance and susceptibility was related to the structure of the peritumoral tissue but not to the cortical structure. The former had 2 ~ 4 layers of pericytes, 36.0-100.8um ;. A high degree of susceptible type - mostly pericyte cells, peritoneal tissue thickness 14.4-24.0um. In the aspects of physiology and biochemistry, the phenylalanine ammonia-lyase activity of disease-resistant type was higher than the susceptible type.