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为探讨肾衰口服液 (SSOL)的作用机理 ,采用细胞培养与细胞分子学技术相结合方法 ,在大鼠肾小球系膜细胞培养体系中加入该药 ,研究其对系膜细胞 (Mesangial cell,MC)增殖和肿瘤坏死因子(TNF- α)的影响。结果 :SSOL加入 MC培养体系 6 d后 ,MC数 (活细胞个数 )为 4 6 70 0± 3819,明显低于对照组 14750 0± 13919(P <0 .0 1) ;该药抑制 MC增殖作用亦较对照组显著 (P <0 .0 1)。MC上清液中 TNF-α含量对照组为 (8.98± 1.11) mg/ L ,治疗组 (1.6 4± 0 .57) mg/ L ,有非常显著性差异 (P <0 .0 1)。提示 SSOL对肾小球 MC增殖有明显抑制作用。
In order to investigate the mechanism of SSOL, a combination of cell culture and cell molecular techniques was applied to the rat mesangial cell culture system to investigate its effect on mesangial cells. , MC) Effects of proliferation and tumor necrosis factor (TNF-α). RESULTS: After 6 days of SSOL addition to MC culture system, the number of MCs (the number of viable cells) was 4 670 0 3819, which was significantly lower than that of the control group 14750 0 13919 (P < 0.01); this drug inhibited MC proliferation. The effect was also significant compared with the control group (P < 0.01). The content of TNF-α in MC supernatant was (8.98±1.11) mg/L in control group and (1.64±0.57) mg/L in treatment group, with very significant difference (P < 0.01). It is suggested that SSOL significantly inhibits the proliferation of glomerular MC.