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人重组βＮＧＦ／ｐＵＣ１９，经ＨｉｎｄⅢ／ＢａｍＨⅠ双酶切后，获βＮＧＦＤＮＡ片段（３６０ｂｐ）。用随机引物标记法进行Ｄｉｇｏｘｉｇｅｎｉｎ标记，在小鼠颌下腺原位杂交检测ＮＧＦｍＲＮＡ显示出很高的特异性，该探针的制备为ＮＧＦ在神经及非神经系统的研究提供新的方法。
Human recombinant βNGF / pUC19, Hind Ⅲ / BamH double digestion after βNGFDNA fragment (360bp). Digoxigenin labeling by random primer method showed high specificity for detecting NGF mRNA by in situ hybridization in mouse submandibular gland. The preparation of this probe provides a new method for the study of NGF in both nervous and non-nervous system.