Wal-Mart’s Darwinism deserves our attention. Aiming at the problems caused by Wal-Mart, this article gives a profound analysis on the concept and model of the enterprise ecosystem and expounds basic characters (such as integrity, hierarchy, functionality, autoregulation etc.) with relevant theories and knowledge of systematology and ecology, and puts forward ecological principles for the construction of harmonious enterprises’ ecosystem (such as principle of interaction, principle of co-evolution, principle of ecoflow, principle of ecological niche, principle of limiting factor and principle of ecological equilibrium) in order to provide new ideas for enterprises’ operation decisions and to promote the whole enterprise ecosystem to develop more healthily, harmoniously and sustainably. Wal-Mart’s Darwinism deserves our attention. Aiming at the problems caused by Wal-Mart, this article gives a profound analysis on the concept and model of the enterprise ecosystem and expounds basic characters (such as integrity, hierarchy, functionality, autoregulation etc.) with relevant theories and knowledge of systematology and ecology, and puts forward ecological principles for the construction of harmonious enterprises’ ecosystem (such as principle of interaction, principle of co-evolution, principle of ecoflow, principle of ecological niche, principle of limiting factor and principle of ecological equilibrium) in order to provide new ideas for enterprises’ operation decisions and to promote the whole enterprise ecosystem to develop more healthily, harmoniously and sustainably.