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重组质粒pJSB13由载体pGEM3多克隆位点中插入人IL-2受体α链cDNA12-937bp片段构成。本文报导了利用载体上的SP6启动子,在体外合成了与插入cDNA片段互补的正链RNA,并用帽子结构类似物在此RNA 5′端进行了加帽,此加帽RNA能在兔网织红细胞体外翻译体系和爪蟾卵母细胞中有效翻译,且翻译产物能分泌到卵母细胞培养液中。
The recombinant plasmid pJSB13 was constructed by inserting the human IL-2 receptor α chain cDNA12-937bp fragment into the multi-cloning site of vector pGEM3. This paper reports that a positive strand RNA complementary to the inserted cDNA fragment was synthesized in vitro using the SP6 promoter on a vector and capped at the 5’end of the RNA with a cap analogue, Erythrocytes in vitro translation system and Xenopus oocytes, and translation products can be secreted into the oocyte culture medium.