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目的探讨PI3K/AKT/m TOR信号转导通路中PTEN、p-AKT和p-m TOR蛋白在胃癌中的表达及其与临床病理特征的关系。方法应用免疫组化法检测胃癌、癌旁和正常胃黏膜组织中PTEN、p-AKT和p-m TOR蛋白的表达情况,并分析其与病理参数之间的关系。结果 PTEN在胃癌中的表达率为53.03%,在癌旁与正常胃黏膜中的表达率为84.85%和74.36%,p-AKT在胃癌中的表达率为56.06%,在癌旁与正常胃黏膜中的表达率为16.67%和2.56%,p-m TOR在胃癌中的表达率为90.91%,在癌旁和正常胃黏膜中的表达率分别为71.21%和5.13%,3组间差异均有统计学意义(P<0.001)。PTEN的阳性表达与患者年龄、性别、肿瘤的直径、分化程度、浸润深度、淋巴结转移以及TNM分期均无关(P>0.05)。p-AKT在胃癌中的表达与患者年龄、性别和肿瘤直径无关(P>0.05),而与肿瘤分化程度、浸润深度、淋巴结转移和TNM分期有关(P<0.05)。p-m TOR在胃癌中的表达仅与肿瘤浸润深度有关(P<0.05),与患者年龄、性别、肿瘤的直径、分化程度、淋巴结转移以及TNM分期均无关(P>0.05)。PTEN蛋白与p-AKT蛋白阳性表达率之间存在负相关关系且具有统计学意义(P<0.05),p-m TOR蛋白与PTEN蛋白以及p-m TOR蛋白与p-AKT蛋白表达阳性率之间均无明显相关(P>0.05)。结论 PTEN在胃癌组织中低表达,p-AKT和p-m TOR在胃癌组织中高表达,PI3K/AKT/m TOR信号转导通路可能参与了胃癌的发生与发展。
Objective To investigate the expression of PTEN, p-AKT and p-m TOR in PI3K / AKT / m TOR signaling pathway and its relationship with clinicopathological features. Methods The expressions of PTEN, p-AKT and p-m TOR in gastric cancer, paracancer and normal gastric mucosa were detected by immunohistochemistry. The relationship between them and the pathological parameters was analyzed. Results The positive rate of PTEN was 53.03% in gastric cancer, 84.85% in normal gastric mucosa and 74.36% in normal gastric mucosa. The positive rate of p-AKT in gastric cancer was 56.06% The expression rates of pm TOR in gastric cancer were 90.91%, 71.21% and 5.13% respectively in paracancer and normal gastric mucosa, the differences among the three groups were statistically significant Significance (P <0.001). The positive expression of PTEN had no correlation with age, sex, tumor diameter, differentiation, depth of invasion, lymph node metastasis and TNM staging (P> 0.05). The expression of p-AKT in gastric cancer was not related to the patient’s age, sex and tumor diameter (P> 0.05), but correlated with tumor differentiation, depth of invasion, lymph node metastasis and TNM staging (P <0.05). The expression of p-m TOR in gastric cancer was only related to the depth of tumor invasion (P <0.05), but not to the age, sex, tumor diameter, differentiation, lymph node metastasis and TNM stage (P> 0.05). There was a negative correlation between the expression of PTEN protein and p-AKT protein (P <0.05), but there was no significant difference between pm TOR protein, PTEN protein, p-AKT protein and pm TOR protein Related (P> 0.05). Conclusions PTEN is overexpressed in gastric cancer tissues, and p-AKT and p-m TOR are highly expressed in gastric cancer tissues. The PI3K / AKT / m TOR signaling pathway may be involved in the development and progression of gastric cancer.