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背景血小板与止血和炎症过程有关,近期的研究已证实血小板促进免疫和炎症主要通过CD40／CD40L途径。本文的目的是研究浓缩血小板在保存期间细胞活素物的聚集。研究设计与方法从血浆分离制备混合浓缩血小板,再悬浮于临床使用级介质中保存;于保存的0,1,2,3和5天时取样分析,无补充(即无可溶蛋白稀释)。用特异的酶联免疫吸附法测定白细胞介素(IL)-6、IL-8、血小板衍生的生长因子(PDGF)-AA、可溶的CD40配体(sCD40L)、RANTES和转化生长因子-β的产生。结
Background Platelets are associated with hemostasis and inflammatory processes, and recent studies have demonstrated that platelets promote immunity and inflammation primarily through the CD40 / CD40L pathway. The purpose of this paper is to investigate the accumulation of cytokines during platelet concentrates. Study Design and Methods Mixed, concentrated platelets were prepared from plasma and resuspended in clinical grade media for storage; sampled at 0, 1, 2, 3, and 5 days of storage with no supplementation (ie no dilution of soluble protein). IL-6, IL-8, platelet-derived growth factor (PDGF) -AA, soluble CD40 ligand (sCD40L), RANTES and transforming growth factor-β were measured by specific enzyme-linked immunosorbent assay The production. Knot