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从金沙江干热河谷地区采集芒果畸形病组织,运用柯赫氏法则证实分离物MG6为该病的致病菌。菌株MG6在马铃薯蔗糖琼脂培养基(PSA)上菌丝白色,无色素产生,米饭培养基浅粉红色;在康乃馨叶片培养基(CLA)上以单瓶梗或复瓶梗假头状产孢,不产生链状孢子;小型分生孢子卵形或长椭圆形,具0-1个分隔,3.1-10.2×1.5-2.2μm;大型分生孢子呈镰刀形,通常3个分隔,18-38×1.8-2.4μm。EF-1α测序结果在Fusarium数据库中进行同源性分析显示,菌株MG6与F.mangiferae的同源性最高,达99.68%。综合培养性状、形态学特征和EF-1α序列分析,将菌株MG6鉴定为Fusarium mangiferae。
The mangrove malformation tissue was collected from the dry-hot valley of the Jinsha River. Koch’s law was used to confirm that the isolate MG6 was the causative agent of the disease. The strain MG6 was hyphae on the potato sucrose agar medium (PSA) and had no pigment. The medium of rice was light pink. Single bottle or bottle ampulla was falsely sporulated on the carnage leaf medium (CLA) No conidia spores; small conidial oval or oblong, with 0-1 partitions, 3.1-10.2 × 1.5-2.2μm; large conidia sickle-shaped, usually three separated, 18-38 × 1.8-2.4 μm. Homology analysis of EF-1α sequencing results in Fusarium database showed that strain MG6 had the highest homology with F. mangiferae, reaching 99.68%. The strain MG6 was identified as Fusarium mangiferae by comprehensively cultivating traits, morphological characteristics and EF-1α sequence analysis.