论文部分内容阅读
妊娠期母体长期暴露于低氧环境可引起胎儿宫内发育迟缓(IUGR)。长期暴露于低氧环境可增强内皮素-1(ET-1)基因表达,使血ET-1水平升高,ET-1主要与受体ET_A结合发挥缩血管效应。为探讨ET在缺氧诱导IUGR中的关键作用,选择Sprague-Dawley雌性小鼠,实验及对照组各12只,于左颈静脉置管以6mg/kg/d分别注入生理盐水及ET_A拮抗剂ER139317;由右颈动脉插管至降主动脉,以采血样行血气分析。小鼠总孕期为22天,在妊娠第18天到第21天,将两组小鼠分别放在正常及低氧(氧含量14％)的实验环境中饲养,然后测小鼠体重,取血样行血气分析,切除子宫称胎鼠及胎盘重量,将获取
Long-term exposure of pregnant mothers during pregnancy hypoxia can cause intrauterine growth retardation (IUGR). Long-term exposure to hypoxia can enhance the expression of endothelin-1 (ET-1), increase the level of ET-1, and ET-1 plays a vasoconstrictive effect mainly in combination with ET_A. In order to explore the key role of ET in hypoxia-induced IUGR, 12 female Sprague-Dawley mice were randomly divided into control group and experimental group. Rats in the left jugular vein were infused with 6 mg / kg / d saline and ET_A antagonist ER139317 ; From the right carotid artery to the descending aorta, blood sampling for blood gas analysis. The total gestational age of the mice was 22 days. From the 18th day to the 21st day of gestation, the two groups of mice were housed in normal and hypoxia (oxygen content 14%) respectively. Then the mice were weighed and the blood samples Line blood gas analysis, removal of the uterus, said fetal rat and placental weight, will be obtained