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目的 观察猴病毒40（SV40）DNA及大T抗原（Tag）在人脑胶质瘤的定位表达情况,探讨其在胶质瘤发生发展中的生物学意义。方法 采用原位杂交和免疫组化技术,检测256例人脑胶质瘤和11例正常脑组织标本。结果 SV40 DNA定位于胶质瘤细胞核,阳性细胞呈弥漫或片灶状分布。胶质瘤SV40 DNA阳性率40.6％（104/256）,正常脑组织均未检测出SV40 DNA,SV40 DNA阳性率与病理分级无关;Tag表达分布与SV40 DNA一致,Tag阳性率28.5％（73/256）较SV40 DNA感染率为低,但Tag表达与病理分级呈显著正相关（P<0.01）。结论SV40感染与人脑胶质瘤病因学密切相关,Tag可能是SV40在胶质瘤发生发展中起作用的重要因素,其机制及生物学意义需进一步研究。
Objective To observe the localization of SV40 DNA and large T antigen in human glioma and to explore its biological significance in the development of glioma. Methods In situ hybridization and immunohistochemistry were used to detect 256 human gliomas and 11 normal brain tissues. Results SV40 DNA was localized in the nucleus of glioma cells. The positive cells were diffuse or focal distribution. The positive rate of SV40 DNA in gliomas was 40.6% (104/256). No SV40 DNA was detected in normal brain tissues. The positive rate of SV40 DNA was not correlated with the pathological grade. The Tag was identical with SV40 DNA in 28.5% 256) was lower than that of SV40 DNA, but there was a significant positive correlation between Tag expression and pathological grade (P <0.01). Conclusion SV40 infection is closely related to the etiology of human glioma. Tag may play an important role in the development of glioma, and its mechanism and biological significance need to be further studied.