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小量过氧化氢（Ｈ＿２Ｏ＿２）预处理，能诱导牛肺动脉内皮细胞（ＢＰＡＥＣｓ）中热休克蛋白７０（ＨＳＰ７０）及ＨＳＰ７０ｍＲＮＡ增多，同时使ＢＰＡＥＣｓ获得了对大量Ｈ＿２Ｏ＿２损伤的耐受性，表现为大量Ｈ＿２Ｏ＿２所致乳酸脱氢酶释放和硫代巴比妥酸反应物含量增加及过氧化氢酶、超氧化物歧化酶活性降低等变化减轻。用放线菌酮和放线菌素Ｄ分别抑制ＨＳＰ７０和ＨＳＰ７０ｍＲＮＡ的增多后，此种耐受性消失。说明小量Ｈ＿２Ｏ＿２预处理使ＢＰＡＥＣｓ对大量Ｈ＿２Ｏ＿２损伤作用产生耐受性与细胞内ＨＳＰ７０合成（包括转录和翻译两个水平）增加及抗氧自由基能力增强有关。
A small amount of H 2 O 2 pretreatment could induce the increase of heat shock protein 70 (HSP70) and HSP70mRNA in bovine pulmonary artery endothelial cells (BPAECs), and at the same time make BPAECs tolerant to a large amount of H 2 O 2 injury, showing a large amount of H 2 O 2 Resulting in the release of lactate dehydrogenase and thiobarbituric acid reactants increased and catalase, superoxide dismutase activity decreased and other changes alleviate. With cycloheximide and actinomycin D inhibit HSP70 and HSP70 mRNA increased, the tolerance disappeared. These results suggest that the pretreatment of H 2 O 2 with a small amount of H 2 O 2 can make the tolerance of BPAECs to a large amount of H 2 O 2 to be related to the increase of intracellular HSP70 synthesis (including transcription and translation) and the enhancement of antioxidant capacity.