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应用胆固醇生物合成的体外酶反应筛选系统，从１０００多株真菌中筛选出具有抑制胆固醇生物合成活性的ＳＩＰＩ－８９２６菌株；对ＳＩＰＩ－８９２６菌株代谢产物研究，应用溶媒萃取、硅胶柱分离、ＬＨ２０凝胶层析和重结晶等方法，从其发酵液中分离出活性成分ＳＩＰＩ－８９２６－Ⅳ。通过元素分析和质谱，确定其分子式为：Ｃ３０Ｈ５０Ｏ３（分子量为：４５８．３７６），综合紫外光谱、红外光谱、１Ｈ－ＮＭＲ和１３Ｃ－ＮＭＲ等图谱数据的解析，确定ＳＩＰＩ－８９２６－Ⅳ为三萜类化合物，结构与中药来源的大豆黄醇Ｂ（ｓｏｙａｓａｐｏｇｅｎｏｌＢ）一致，化学命名为：３β，２１β，２４－三羟基－齐果墩烷－１２－烯
In vitro enzyme reaction screening system of cholesterol biosynthesis, SIPI-8926 strain with inhibitory activity of cholesterol biosynthesis was screened from more than 1000 strains of fungi. The metabolites of SIPI-8926 strain were studied by solvent extraction, silica gel column, LH20 condensate Gel chromatography and recrystallization, the active ingredient SIPI-8926-IV was isolated from the fermentation broth. The molecular formula of C30H50O3 (molecular weight: 458.376) was determined by elemental analysis and mass spectrometry. The analysis of the data by UV, IR, 1H-NMR and 13C-NMR showed that SIPI-8926- Class compounds, the structure and the source of Chinese medicine soybean glycolate B (soyasapogenolB), chemical name: 3β, 21β, 24-trihydroxy - Qi fruiting 12-ene