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采用世界卫生组织（ＷＨＯ） 推荐使用的方法，用Ｌ２０Ｂ、ＲＤ和Ｈｅｐ２ 三种细胞进行脊髓灰质炎（脊灰）病毒的分离鉴定。结果表明，Ｌ２０Ｂ细胞具有良好的特异性，对非脊灰肠道病毒均不敏感。在脊灰分离株和粪便悬液标本中脊灰病毒的检出率分别为１００％ 和９３－３ ％ ，优于ＲＤ和Ｈｅｐ２ 细胞，特别是在病毒分布不均及混有非脊灰肠道病毒的情况下，能更好地、准确地捕捉到脊灰病毒。采用Ｌ２０Ｂ加ＲＤ细胞分离脊灰病毒优于ＲＤ加Ｈｅｐ２，但对非脊灰肠道病毒分离率有影响。
Using the method recommended by the World Health Organization (WHO), L20B, RD and Hep 2 were used to isolate and identify poliomyelitis (polio) virus. The results showed that L20B cells have good specificity and are not sensitive to non-polio enterovirus. The detection rate of poliovirus in poliovirus isolates and fecal samples were 100% and 93-3%, respectively, which were better than that of RD and Hep2 cells, especially in the case of uneven distribution of virus and mixed with non-poliovirus In the case of enterovirus, poliovirus can be captured more accurately and accurately. The use of L20B plus RD cells isolated poliovirus is superior to RD plus Hep 2, but non-polio enteroviral isolation rate.