本研究描述了一种从新鲜芒果叶片中分离纯化天然芒果苷的新颖简单且可大量制备的方法.以甲醇作为溶剂,采用超声辅助的方法从芒果叶片中提取芒果苷.芒果苷粗提物以及通过甲醇-氯仿(10∶1,v/v)重结晶得到的产物的得率分别为1.41％(纯度86.5％)和0.75％(纯度大于99.6％).采用紫外分光光度法和高效液相法对芒果苷进行纯度分析.通过UV、IR、Q-TOF/MS、1H NMR和13C NMR对纯化后的化合物结构进行鉴定.本文报道了一种简单、经济、快速、高效的芒果苷纯化方法.同时,采用该方法首次获得纯度超过99.6％的芒果苷.“,”In the present study,we described a novel,simple,and scalable method for isolating and purifying natural mangiferin from fresh mango leaves.An ultrasonic-assisted method was used to extract mangiferin from mango leaves using methanol as the solvent.The yields of the mangiferin crude extract and purified mangiferin recrystallized from a mixture of methanol and trichloromethane(10∶1,v/v)were 1.41％(purity 86.5％)and 0.75％(purity＞99.6％),respectively.The purity of mangiferin was assessed by UV spectroscopy and HPLC.The structure of the purified compound was confirmed spectroscopically by UV,IR,Q-TOF/MS,1H NMR,and 13C NMR.The method described herein to isolate mangiferin was simple,inexpensive,rapid,and efficient.Moreover,we,for the first time,obtained mangiferin with a purity of more than 99.6％using this method.