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目的:了解大鼠体内天然抗体IgM在非协调性异种心脏移植超急性排斥反应中的作用。方法:以培养豚鼠心脏微血管内皮细胞为异种心脏移植的细胞实验模型,通过补体结合实验、免疫组化等方法研究大鼠抗体IgM对该细胞的作用,了解异种抗体IgM在异种移植超急性排斥反应中的作用机制。结果:在该模型中,大鼠抗体IgM可与豚鼠心脏微血管内皮细胞结合并能激活补体,产生对该细胞的损伤,而经小鼠抗大鼠IgM处理后的大鼠血清,对豚鼠心脏微血管内皮细胞的损伤作用明显减轻。结论:在该实验模型中,异种天然抗体IgM是激活补体引起超急性排斥反应的重要因素。
OBJECTIVE: To understand the role of natural antibody IgM in the rat model of hyperacute rejection of nonspecific heterogenous heart transplantation. Methods: The cultured rat cardiac microvascular endothelial cells as a heterologous heart transplantation experimental model of cells by complement fixation experiments, immunohistochemistry and other methods to study the role of rat antibody IgM on the cells to understand the different antibody IgM in the hyperacute rejection of xenotransplantation In the mechanism of action. Results: In this model, rat antibody IgM could bind to guinea pig cardiac microvascular endothelial cells and activate complement, resulting in damage to the cells. However, rat anti-rat IgM serum was administered to guinea pig cardiac microvascular Endothelial injury was significantly reduced. CONCLUSIONS: In this experimental model, the native IgM is an important factor in the activation of complement to cause hyperacute rejection.